e ab f1121e  (Elabscience Biotechnology)

Journal: The Korean Journal of Pain

Article Title: IL-27-Ucp2-FoxO3 axis mediating the polarization of alternatively activated macrophages and ameliorating inflammatory pain

doi: 10.3344/kjp.25307

Figure Lengend Snippet: The identification of the source of IL-27. (A–D) The intracellular co-label staining by flow cytometry was applied to determine the source of IL-27. The four APCs were labeled by dendritic cell (CD11b, CD11c, B), neutrophil (CD11b, Ly6G, C), monocyte (CD11b, Ly6C, A), and macrophage (CD11b, F4/80, D), respectively. IL-27p28 antibody labeled the IL-27. The results indicated that neutrophil/monocyte-derived IL-27 was highly expressed in serum and spleen. IL: interleukin, APCs: antigen-presenting cells, SSC-A: side scatter area.

Article Snippet: APC Anti-Mouse Ly6C , Elabscience , E-AB-F1121E.

Techniques: Staining, Flow Cytometry, Labeling, Derivative Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Modeling pathogen-driven neonatal late-onset sepsis: a modification to the murine cecal slurry

doi: 10.3389/fcimb.2025.1589712

Figure Lengend Snippet: Characterization of pathologic slurry induced sepsis on the relative abundance of leukocytes in the lamina propria and spleen. Four hours following injection, mice were euthanized and single cell suspensions of leukocytes from lamina propria and spleens were processed for flow cytometry as described in methods. (A) Representative gating strategy: Single cell leukocytes were first gated by CD3 and CD19. Among CD3+, CD19- cells, CD4 helper T-cells were defined as CD4+, CD8- and CD8 cytotoxic T cells were defined as CD8+, CD4-. Regulatory T cells were identified as CD4+, CD25+. B cells were defined as CD19+, CD3-. Among CD3-,CD19- cells, Natural Killer cells were defined as NK1.1+, Dendritic cells as MHCII+, CD11c+, Neutrophils as Ly6G+, CD11b+, macrophages as F4/80+, CD11b+ and monocytes as MHCII low , Ly6C+. The relative expression of leukocyte populations within the (B) Lamina Propria (LP) and (C) Spleen was compared between control mice and sepsis exposed mice. Comparisons were made by unpaired T tests. Bars represent the mean values with error bars representing the standard error of the mean. Control LP (N = 12), Sepsis LP (N = 9), Control Spleen (N = 12), Sepsis Spleen (N = 10), significance noted as *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The following antibodies were used for staining: PE-Cy7 conjugated anti-mouse CD3, PE conjugated anti-mouse CD4, FITC conjugated anti-mouse CD8, PE conjugated anti-mouse CD11b, FITC conjugated anti-mouse CD11c, PE-efluor 610 conjugated anti-mouse CD25, APC conjugated anti-mouse Ly6C, PE-Cyanine5 conjugated anti-mouse Ly6G, Alexa Fluor 700 conjugated anti-mouse MHC Class II, Brillian Violet 605 conjugated anti-mouse NK1.1, unconjugated anti-mouse CD16/CD32 (all from eBioscience inc, Santa Clara, CA), APC-Cy7 conjugated anti-mouse CD19 (BD Biosciences, San Jose, CA), PE-Texas Red conjugated anti-mouse F4/80 (Invitrogen, Waltham, MA).

Techniques: Injection, Flow Cytometry, Expressing, Control